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dc.contributor.authorAl-Hakim, Ali
dc.contributor.authorLinhardt, Robert J.
dc.date1990
dc.date.accessioned2022-06-27T17:15:23Z
dc.date.available2022-06-27T17:15:23Z
dc.date.issued1990-01-01
dc.identifier.citationIsolation and Recovery of Acidic Oligosaccharides from Polyacrylamide Gels by Semi-Dry Electrotransfer, A. Al-Hakim, R.J. Linhardt, Electrophoresis, 11, 23-28 (1990).
dc.identifier.issn15222683
dc.identifier.issn1730835
dc.identifier.urihttps://doi.org/10.1002/elps.1150110106
dc.identifier.urihttps://hdl.handle.net/20.500.13015/6009
dc.descriptionElectrophoresis, 11, 23-28
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractAcidic oligosaccharides derived from glycosaminoglycan heparin were separated by polyacrylamide gradient gel electrophoresis (PAGE). The gel could be visualized using Alcian Blue dye to give a pattern of highly resolved, well defined bands. The particular banding pattern obtained was the result of a heparinase catalyzed depolymerization which afforded oligosaccharide products that differed in size by one disaccharide unit. The separated oligosaccharides could be recovered prior to staining by electroelution onto a positively charged nylon membrane by a semi-dry transfer procedure. Subsequent elution and quantitative recovery of individual oligosaccharides from the membrane was achieved. By using multiple membrane layers a second separation dimension was obtained, resulting in increased oligosaccharide purity proportional to transfer depth. Preparative gradient polyacrylamide gel electrophoresis followed by semi-dry electro-transfer and recovery represents a novel method for the preparation of homogeneous acidic oligosaccharides.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1002/elps.1150110106
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofELECTROPHORESIS
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleIsolation and Recovery of Acidic Oligosaccharides from Polyacrylamide Gels by Semi-Dry Electrotransfer
dc.typeArticle
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dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1002/elps.1150110106
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages23-28
rpi.description.volume11


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