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dc.contributor.authorMallis, Larry M.
dc.contributor.authorWang, Hui M.
dc.contributor.authorLoganathan, Duraikkannu
dc.contributor.authorLinhardt, Robert J.
dc.date1989
dc.date.accessioned2022-06-27T17:15:23Z
dc.date.available2022-06-27T17:15:23Z
dc.date.issued1989-01-01
dc.identifier.citationSequence Analysis of Highly Sulfated Heparin-Derived Oligosaccharides Using Fast Atom Bombardment Mass Spectrometry, L.M. Mallis, H.M. Wang, D. Loganathan, R.J. Linhardt, Analytical Chemistry, 61, 1453-1458 (1989).
dc.identifier.issn15206882
dc.identifier.issn32700
dc.identifier.urihttps://doi.org/10.1021/ac00188a030
dc.identifier.urihttps://hdl.handle.net/20.500.13015/6011
dc.descriptionAnalytical Chemistry, 61, 1453-1458
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin, a polydisperse, sulfated copolymer of 1----4 linked glucosamine and uronic acid residues, has been used clinically as an anticoagulant for half a century. Despite a yearly use of over 50 million doses in the U.S. alone, heparin's exact chemical structure remains unclear. The negative ion fast atom bombardment mass spectrometry (FAB-MS) analysis is presented for a series of enzymatically prepared, homogeneous, structurally characterized, highly sulfated, heparin-derived oligosaccharides using triethanolamine as the FAB matrix. In addition to the clear presence of monoanionic sodiated molecular ions, structurally significant (sequence) fragment ions are observed and characterized with respect to the known structure for five of the heparin-derived oligosaccharides. The structure of a sixth oligosaccharide is predicted by using negative ion FAB-MS and subsequently confirmed by chemical, enzymatic, and NMR spectroscopic methods.
dc.description.sponsorshipNational Institute of General Medical Sciences
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/ac00188a030
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAnalytical Chemistry
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleSequence Analysis of Highly Sulfated Heparin-Derived Oligosaccharides Using Fast Atom Bombardment Mass Spectrometry
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/ac00188a030
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1021/ac00188a030
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages1453-1458
rpi.description.volume61


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