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dc.contributor.authorKim, Yeong Shik
dc.contributor.authorLee, Kyung Bok
dc.contributor.authorLinhardt, Robert J.
dc.identifier.citationMicroheterogeneity of Plasma Glycoproteins Antithrombin III and Heparin Cofactor II and Their Carbohydrate Analysis, Y.S. Kim, K.B. Lee and R.J. Linhardt, Thrombosis Research, 51, 97-104 (1988).
dc.descriptionThrombosis Research, 51, 97-104
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractAntithrombin III is the most important protease inhibitor in the blood coagulation cascade. Its activity results from the formation of covalent complexes with Factor Xa and thrombin (FIIa) (1). The rate of this inhibition is accelerated up to 2,000-fold in the presence of catalyst, heparin (1). AT111 is a single-chain glycoprotein reportedly containing 9-17% of carbohydrate (2,3). Multiple isoforms of AT111 have been detected by the isoelectric focusing of plasma and purified protein and this microheterogeneity has been attributed to variable sialation (4-7). Recently, a second heparin cofactor has been identified and purified from plasma (8-10). Heparin cofactor II (HCII) is also a glycoprotein, which is antigenically distinct from AT111 and inhibits only FIIa in the presence of heparin (8). Its carbohydrate structure and isoelectric point have not been reported. We confirm the presence of multiple IEF bands for HCII (8). Unlike AT111 it remains heterogeneous even after desialation. Ion-exchange chromatography with pulsed- amperometric detection is used for the first time, on microgram quantities of glycoprotein, to determine the concentration of each neutral sugar, amino sugar and sialic acid residue. The carbohydrate composition of AT111 and HCII are reported.
dc.description.sponsorshipNational Heart, Lung, and Blood Institute
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofThrombosis Research
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleMicroheterogeneity of Plasma Glycoproteins Antithrombin III and Heparin Cofactor II and Their Carbohydrate Analysis
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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