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dc.contributor.authorRice, K.G.
dc.contributor.authorRottink, M.K.
dc.contributor.authorLinhardt, Robert J.
dc.date1987
dc.date.accessioned2022-06-27T17:15:44Z
dc.date.available2022-06-27T17:15:44Z
dc.date.issued1987-01-01
dc.identifier.citationFractionation of Heparin-Derived Oligosaccharides by Gradient Polyacrylamide-Gel Electrophoresis, K.G. Rice, M.K. Rottink, R.J. Linhardt, Biochemical Journal, 244, 515-522 (1987).
dc.identifier.issn2646021
dc.identifier.urihttps://doi.org/10.1042/bj2440515
dc.identifier.urihttps://hdl.handle.net/20.500.13015/6019
dc.descriptionBiochemical Journal, 244, 515-522
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known structure and having a degree of polymerization (dp) 2-6 were used to establish that the separation on gradient PAGE was primarily dependent on molecular size. High-Mr oligosaccharides (dp 8-20) were prepared using strong-anion-exchange h.p.l.c. and were used to help characterize the gradient PAGE separation. Kinetic profiles were obtained for the depolymerization of heparin and heparan sulphate with heparinase and heparitinase respectively. The utility of this approach in sequencing oligosaccharides derived from glycosaminoglycans is discussed.
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1042/bj2440515
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofThe Biochemical journal
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleFractionation of Heparin-Derived Oligosaccharides by Gradient Polyacrylamide-Gel Electrophoresis
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1042/bj2440515
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1042/bj2440515
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages515-522
rpi.description.volume244


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