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dc.contributor.authorLinhardt, R. J.
dc.contributor.authorCooney, C. L.
dc.contributor.authorTapper, D.
dc.contributor.authorZannetos, C. A.
dc.contributor.authorLarsen, A. K.
dc.contributor.authorLanger, R.
dc.date1984
dc.date.accessioned2022-06-27T17:16:06Z
dc.date.available2022-06-27T17:16:06Z
dc.date.issued1984-02-01
dc.identifier.citationAn Immobilized Microbial Heparinase for Blood Deheparinization, R.J. Linhardt, C.L. Cooney, A. Larsen, C.A. Zannetos, D. Tapper, R. Langer, Applied Biochemistry and Biotechnology, 9, 41-55 (1984).
dc.identifier.issn15590291
dc.identifier.issn2732289
dc.identifier.urihttps://doi.org/10.1007/BF02798373
dc.identifier.urihttps://hdl.handle.net/20.500.13015/6031
dc.descriptionApplied Biochemistry and Biotechnology, 9, 41-55
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in a fluidized bed reactor to eliminate heparin from blood passing through an extracorporeal circuit both in vitro and in vivo. This paper discusses the stepwise development of this heparinase reactor including: (1) improvements in the fermentation resulting in an inexpensive large-scale source of heparinase without the addition of the previously required inducer, heparin; (2) the use of batch processes to adapt previous purification schemes to large-scale heparinase production and the subsequent purification of heparinase to a single SDS-PAGE banding protein; (3) the immobilization of heparinase with a 91% activity recovery and good stability, (4) the design and successful testing of a fluidized bed reactor containing immobilized heparinase in the removal of clinically used quantities of heparin from both human blood in vitro and canine blood in vivo; and (5) the initiation of animal studies focusing on the toxicology of heparinase-derived heparin degradation products and the short and long term effects of exposure to these products and to heparinase.
dc.description.sponsorshipNational Institute of General Medical Sciences
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/BF02798373
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleAn Immobilized Microbial Heparinase for Blood Deheparinization
dc.typeArticle
dcterms.accessRightshttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1007/BF02798373
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1007/BF02798373
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages41-55
rpi.description.volume9


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