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    A System for Heparin Removal

    Author
    Langer, R.; Linhardt, Robert J.; Klein, M.; Galliher, P.M.; Cooney C.L.; Flanagan, M.M.
    ORCID
    https://orcid.org/0000-0003-2219-5833
    Thumbnail
    Other Contributors
    Date Issued
    1982
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    A System for Heparin Removal, R. Langer, R.J. Linhardt, M. Klein, P.M. Galliher, C.L. Cooney and M.M. Flanagan, Biomaterials: Inter-facial Phenomenon and Applications, Advances in Chemistry Symposium Series, Chapter 31, pp. 493-509, S. Cooper, A. Hoffman, N. Pepas and B. Ratner (Eds.), Washington, D.C. 1982.
    Metadata
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    URI
    https://doi.org/10.1021/ba-1982-0199.ch031; https://hdl.handle.net/20.500.13015/6042
    Abstract
    Extracorporeal medical machines rely on systemic heparinization to improve blood compatibility. However, heparin can lead to serious complications such as hemorrhaging. We propose a new approach to control heparin levels by employing a blood filter containing immobilized heparinase. Such a filter could potentially enable heparinization of an extracorporeal circuit without simultaneous heparinization of the patient. The principal findings of our work thus far include (1) increasing volumetric enzyme production over 1000-fold from previously published procedures; (2) purifying heparinase by over 1000-fold from the crude cell extracts; (3) characterizing the biochemical properties of heparinase; (4) isolating the first heparinase inhibitors; (5) immobilizing heparinase with 91% activity recovery and excellent stability; and (6) demonstrating that columns as small as 1.5 mL can remove clinically used quantities of heparin in aqueous medium and in blood.;
    Description
    Biomaterials: Inter-facial Phenomenon and Applications, Advances in Chemistry Symposium Series, Chapter 31, pp. 493-509, S. Cooper, A. Hoffman, N. Pepas and B. Ratner (Eds.), Washington, D.C.; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
    Access
    https://login.libproxy.rpi.edu/login?url=https://doi.org/10.1021/ba-1982-0199.ch031;
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