Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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A System for Heparin Removal, R. Langer, R.J. Linhardt, M. Klein, P.M. Galliher, C.L. Cooney and M.M. Flanagan, Biomaterials: Inter-facial Phenomenon and Applications, Advances in Chemistry Symposium Series, Chapter 31, pp. 493-509, S. Cooper, A. Hoffman, N. Pepas and B. Ratner (Eds.), Washington, D.C. 1982.
Extracorporeal medical machines rely on systemic heparinization to improve blood compatibility. However, heparin can lead to serious complications such as hemorrhaging. We propose a new approach to control heparin levels by employing a blood filter containing immobilized heparinase. Such a filter could potentially enable heparinization of an extracorporeal circuit without simultaneous heparinization of the patient. The principal findings of our work thus far include (1) increasing volumetric enzyme production over 1000-fold from previously published procedures; (2) purifying heparinase by over 1000-fold from the crude cell extracts; (3) characterizing the biochemical properties of heparinase; (4) isolating the first heparinase inhibitors; (5) immobilizing heparinase with 91% activity recovery and excellent stability; and (6) demonstrating that columns as small as 1.5 mL can remove clinically used quantities of heparin in aqueous medium and in blood.;
Biomaterials: Inter-facial Phenomenon and Applications, Advances in Chemistry Symposium Series, Chapter 31, pp. 493-509, S. Cooper, A. Hoffman, N. Pepas and B. Ratner (Eds.), Washington, D.C.; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;