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dc.contributor.authorGalliher, P.M.
dc.contributor.authorCooney, C.L.
dc.contributor.authorLanger R.
dc.contributor.authorLinhardt, Robert J.
dc.date1981
dc.date.accessioned2022-06-27T17:16:34Z
dc.date.available2022-06-27T17:16:34Z
dc.date.issued1981
dc.identifier.citationHeparinase Production by Flavobacterium heparinum, P.M. Galliher, C.L. Cooney, R. Langer and R.J. Linhardt, Applied and Environmental Microbiology, 41, 360-365 (1981).
dc.identifier.urihttps://doi.org/10.1128/aem.41.2.360-365.1981
dc.identifier.urihttps://hdl.handle.net/20.500.13015/6044
dc.descriptionApplied and Environmental Microbiology, 41, 360-365
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter per h in the complex medium. Rapid deactivation of heparinase activity, both specific and total, was observed at the onset of the stationary phase. Nutritional studies on growth and heparinase production showed an obligate requirement for L-histidine and no vitamin requirement. L-Methionine partially relieved the L-histidine requirement. A defined medium containing glucose, ammonium sulfate, basal salts, L-methionine, and L-histidine was developed for growth and heparinase production. The growth rate in this medium was 0.21 h-1, which is 40%, higher than that in complex medium. The maximum volumetric productivity of heparinase in the defined medium was increased to 1,475 U/liter per h, providing a 640-fold increase over that achieved by previously published methods. No rapid deactivation was observed. An examination of alternate inducers for heparinase showed that heparin degradation products, hyaluronic acid, heparin monosulfate, N-acetyl-D-glucosamine, and maltose, induce heparinase in complex medium. An Azure A assay was modified and fully developed to measure the heparin concentration during fermentation and the heparinase specific activity of crude extracts of F. heparinum obtained from sonication, thus negating the need for further purification to measure activity."
dc.description.urihttps://login.libproxy.rpi.edu/login?url=https://doi.org/10.1128/aem.41.2.360-365.1981
dc.languageen_US
dc.language.isoENG
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHeparinase Production by Flavobacterium heparinum
dc.typeArticle
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dcterms.isVersionOfhttps://doi.org/10.1128/aem.41.2.360-365.1981
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dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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