Quantitative analysis of heparan sulfate using isotopically labeled calibrants
Authors
Liu, J.
Wang, Z.
Arnold, K.
Xu, Y.
Pagadala, V.
Su, G.
Myatt, H.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2020
Keywords
Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering , Biology
Degree
Terms of Use
Attribution 3.0 United States
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In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
Full Citation
Developing a quantitative method to analyze heparan sulfate using isotopically labeled calibrants, Z. Wang, K. Arnold, Y. Xu, V. Pagadala, G. Su, H. Myatt, R.J. Linhardt, J. Liu, Communications Biology, 3, 425, 2020.
Abstract
Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
Description
Communications Biology, 3, 425
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
Rensselaer Polytechnic Institute, Troy, NY
The Linhardt Research Labs Online Collection
https://harc.rpi.edu/
The Linhardt Research Labs Online Collection
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI