Chemical Microdetermination of Heparin in Plasma
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Authors
Toida, Toshihiko
Huang, Yong
Washio, Yukio
Maruyama, Tomohiro
Toyoda, Hidenao
Imanari, Toshio
Linhardt, Robert J.
Issue Date
1997-09-05
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A new, simple, and highly sensitive method for the determination of heparin has been established. Heparin was first converted into unsaturated disaccharides through the action of heparin lyases I, II, and III. A major trisulfated unsaturated disaccharide product results, consistent with structural analysis of a number of pharmaceutical heparins using one- and two-dimensional 1H NMR spectroscopy. This disaccharide was analyzed by HPLC using fluorometric postcolumn derivatization. The correlation between the amount of this trisulfated unsaturated disaccharide and anticoagulant activity of heparin as measured by anti-IIa was determined. The analysis of these pharmaceutical heparins showed a linear correlation between both HPLC and bioassay. This HPLC method was then applied to a pharmacokinetic study of heparin intravenously administered to rabbits.
Description
Analytical Biochemistry, 251, 219-226
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Chemical Microdetermination of Heparin in Plasma T. Toida, Y.Huang, T. Maruyama, H. Toyoda, T. Imanari, R.J. Linhardt, AnalyticalBiochemistry, 251, 219-226, 1997.
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Volume
Issue
PubMed ID
DOI
ISSN
32697