Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding

Weyers, Amanda
Yang, Bo
Solakyildirim, Kemal
Yee, Vienna
Li, Lingyun
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding,A. Weyers, B. Yang, K. Solakyildirim, V. Yee, L. Li, F. Zhang, R. J. Linhardt, FEBS Journal, 280, 2285–2293, 2013.
Keratan sulfate (KS) is an important glycosaminoglycan that is found in cartilage, reproductive, and neural tissues. Corneal KS glycosaminoglycan is found N-linked to lumican, keratocan, and mimecan proteoglycans and has been widely studied by investigators interested in corneal development and diseases. Recently, the availability of corneal KS has become severely limited due to restricted the shipment of bovine central nervous system by-products across international borders in efforts to prevent additional cases of mad cow disease. We report a simple method for the purification of multi-milligram quantities of bovine corneal KS and characterize its structural properties. We also examined its protein-binding properties and discovered that corneal KS bound with high affinity to fibroblast growth factor-2 and sonic hedgehog, a growth factor and a morphogen involved in corneal development and healing.
FEBS Journal, 280, 2285–2293
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
FEBS Press
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Rensselaer Polytechnic Institute, Troy, NY
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