Development of DNA toehold switch based Zika virus sensor

Authors
Jimcosky, Kara
ORCID
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Other Contributors
Wang, Xing
McGown, Linda Baine
Linhardt, Robert J.
Issue Date
2019-05
Keywords
Chemistry
Degree
MS
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
The Zika virus (ZIKV) is a flavivirus known to cause several birth defects such as microcephaly and underdeveloped neural tissue in unborn babies of infected mothers. Individuals with ZIKV also have a risk of contracting severe Guillain-Barre Syndrome, an autoimmune disease that destroys nerve cells and can cause paralysis. Current methods for ZIKV detection include IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) and Reverse-Transcriptase PCR (RT-PCR). Cross-reactivity of the IgM antibodies with other flaviviruses such as Dengue virus (DENV) and Chikungunya requires additional confirmation via plaque-reduction neutralization testing (PRNT), an expensive, and complex process. This work presents a unique, DNA-based method of detection which is specific, sensitive, simple, cost effective, and could ultimately become field applicable.
Description
May 2019
School of Science
Department
Dept. of Chemistry and Chemical Biology
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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