Analysis of heparin oligosaccharides by capillary electrophoresis- negative-ion electrospray ionization mass spectrometry
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Authors
Lin, Lei
Liu, Xinyue
Zhang, Fuming
Chi, Lianli
Amster, I. Jonathan
Leach, Franklyn E.
Xia, Qiangwei
Linhardt, Robert J.
Issue Date
2017-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Most hyphenated analytical approaches that rely on liquid chromatography–MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization. Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE–MS coupling eletrospray ion source. Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid. The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin. The application of this CE–MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
Description
Analytical and Bioanalytical Chemistry,409, 411–420
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Analysis of heparin oligosaccharides by capillary electrophoresis- negative-ion electrospray ionization mass spectrometry, L. Lin, X. Liu, F. Zhang, L. Chi, I. J. Amster, F. E. Leach III, Q. Xia, R. J. Linhardt, Analytical and Bioanalytical Chemistry,409, 411–420, 2017.
Publisher
Springer
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
16182650
16182642
16182642