Monoclonal Antibodies Prepared Against Heparin Lyase I and their Reactivity Towards Heparin Lyases I, II and III

Authors
Gu., K.
Edens, R.E.
Jandik, K.A.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
1993
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Monoclonal Antibodies Prepared Against Heparin Lyase I and their Reactivity Towards Heparin Lyases I, II and III, K. Gu., R.E. Edens, K.A. Jandik, R.J. Linhardt, International Journal of Biochemistry, 25, 331-336 (1993).
Abstract
1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and by Western blotting. 3. Individual antibodies showed different reactivity toward the three heparin lyases. 4. The reactivity of two of the monoclonal antibodies was destroyed by exposing heparin lyases to sodium dodecyl sulfate. 5. The antibodies can be used to rapidly distinguish between the three heparin lyases.
Description
International Journal of Biochemistry, 25, 331-336
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
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