Na+ transporters critical for infection and adaptation of Pseudomonas aeruginosa in Drosophila melanogaster

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Authors
Joseph, Gemel Amillia Georgette
Issue Date
2020-05
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Electronic thesis
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ENG
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Biology
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Abstract
We found that the Na+-NQR deletion produced no change in the bacterium’s ability to kill the flies, while the Mrp deletion produced a significantly reduced virulent rate in the bacterium. The reason for altered infectivity was not due to the altered pyocyanin production or biofilm formation because there was no observed correlation with mortality. Bacterial load assays measuring viable P. aeruginosa in the infected fly showed that the Mrp deletion mutant was unable to grow as well in the fly as wild type and the Na+-NQR mutant. We conclude that the ability to kill is dependent on the ability to survive and grow in the host environment as Drosophila mortality was correlated with bacterial growth in the fly. These findings support Mrp’s role in Na+ regulation as crucial in infection and colonization in Drosophila.
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May 2020
School of Science
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Rensselaer Polytechnic Institute, Troy, NY
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