Microscale separation of heparosan, heparan sulfate and heparin
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Authors
Zhao, Xue
Yang, Bo
Linkens, Kathryn
Datta, Payel
Onishi, Akihiro
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2013-03-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in glycomics analysis. A method for microscale separation and quantification of heparin, heparan sulfate and heparosan from cells is reported. This separation relies on a mini-strong anion exchange spin column eluted stepwise with different concentrations of sodium chloride. Disaccharide analysis by LC-MS was used to monitor the chemical structure of the different GAG chains that were recovered.
Description
Analytical Biochemistry, 434, 215–217
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Microscale separation of heparosan, heparan sulfate and heparin, X. Zhao, B. Yang, K. Linkens, P. Datta, A. Onishi, F. Zhang, R. J. Linhardt, Analytical Biochemistry, 434, 215–217, 2013.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697