The influence of cellular patterning on brown adipogenic differentiation from mouse embryonic stem cells

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Authors
Dias, Andrew
Issue Date
2015-08
Type
Electronic thesis
Thesis
Language
ENG
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Biomedical engineering
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Abstract
Embryonic stem cells can theoretically have regenerative medicine applications for any tissue type, because they can self-renew, and have the potential to differentiate into any somatic cell type. However, harnessing this potential remains challenging, as stem cell fate decisions to specific cell types are difficult to control. Many have previously used morphogens and engineered substrates to direct differentiation, but another aspect of the stem cell microenvironment, that has received considerably less attention, is cellular interactions and signaling. Cell patterning can potentially provide some degree of control over cellular signaling by cell-cell contacts, juxtacrine, and paracrine signaling. The goal of this thesis was to pattern mouse embryonic stem cells (mESCs) using laser direct-write (LDW), and evaluate the influence of patterning on their differentiation.
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August 2015
School of Engineering
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Rensselaer Polytechnic Institute, Troy, NY
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