[[The]] role of the bacterial ribosome in barotolerance

Authors
Smith, Walter P.
ORCID
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Other Contributors
Landau, Joseph V.
Pope, Daniel H.
Ehrlich, Henry Lutz, 1925-
Boylen, Charles W.
Hurwitz, Charles
Aronson, John N.
Issue Date
1976-08
Keywords
Molecular biology
Degree
PhD
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This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
Examination of the pressure response of the protein synthesizing systems utilizing all the possible 30s and 50s ribosomal subunit combinations from E. coli, P. fluorescens and P. bathycetes indicated that the salt-mediated barotolerant characteristic associated with P. bathycetes ribosomes lies within the functional capabilities of the 3Os subunit. Furthermore, the concentration of selected ions utilized in the process of separation and isolation of P. bathycetes ribosomal subunits was a critical factor. Utilization of isolation media containing either ImM Mg++, ImM Mg++ plus 150mM Na+, or lmM Mg++ plus 150mM K+ resulted in particles being obtained which were either respectively unable to synthesize polypeptide, capabLe of polypeptide synthesis which was barosenstive at both low and high salts, or capable of synthesis which was barosensitive in low salts and barotolerant in high salts. The inability to synthesize polypeptide has been shown to be due to an in ability to form messenger RNA-ribosomecampLexes , Furthermore riboosome isolated in 1mM Mg++ salts display altered sedimentation profiles, i.e. 30s particles appear as 26s particles. This change in s value may be due to the loss of specific ribosomal proteins during the isolation proceedure. The loss of the barotolerant characteristic may be due to specific ion-macromolecular interactions whLch cause irreversible conformational changes in the 30s ribosomal subunit.
Description
August 1976
School of Science
Department
Dept. of Bioinformatics and Molecular Biology
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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