Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry
No Thumbnail Available
Authors
Bazin, H.G.
Wolff, Michael W.
Linhardt, Robert J.
Issue Date
1999-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.
Description
Biotechnology Techniques,13,797-801
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometryH. G. Bazin, M.W. Wolff, R.J. Linhardt, Biotechnology Techniques,13,797-801, 1999.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
0951208X