Characterization of human placental glycosaminoglycans and regional binding to VAR2CSA in malaria infected erythrocytes
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Authors
Beaudet, Julie M.
Mansur, Leandra
Joo, Eun Ji
Kamhi, Eyal
Yang, Bo
Clausen, Thomas M.
Salanti, Ali
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2014-02-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Placental malaria is a serious problem in sub-Saharan Africa. Young women are particular susceptible to contracting this form of malaria during their first or second pregnancy despite previously acquired immunity from past infections. Placental malaria is caused by Plasmodium falciparum parasites expressing VAR2CSA on the erythrocyte surface. This protein adheres to a low-sulfated chondroitin sulfate-A found in placental tissue causing great harm to both mother and developing fetus. In rare cases, the localization of infected erythrocytes to the placenta can even result in the vertical transmission of malaria. In an effort to better understand this infection, chondroitin sulfate was isolated from the cotyledon part of the placenta, which should be accessible for parasite adhesion, as well as two non-accessible parts of the placenta to serve as controls. The placental chondroitin sulfate structures and their VAR2CSA binding were characterized. All portions of human placenta contained sufficient amounts of the appropriate low-sulfated chondroitin sulfate-A to display high-affinity binding to a recombinant truncated VAR2CSA construct, as determined using surface plasmon resonance. The cotyledon is the only placental tissue accessible to parasites in the bloodstream, suggesting it is the primary receptor for parasite infected red blood cells.
Description
Glycoconjugate Journal, 31, 109–116
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Characterization of human placental glycosaminoglycans and regional binding to VAR2CSA in malaria infected erythrocytes, J. M. Beaudet, L. Mansur, E.-J. Joo, E. Kamhi, B. Yang, T. M Clausen, A. Salanti, F. Zhang, R. J. Linhardt, Glycoconjugate Journal, 31, 109–116, 2014.
Publisher
Springer
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15734986
2820080
2820080