The microbial degradation of a cutting oil concentrate
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Authors
Pierce, George E.
Issue Date
1976-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biology
Alternative Title
Abstract
Using cultures of E. oleovorans.the concentration of fatty acids has been shown to increase with time. As the concentration of fatty acids increases, the rate of saturate degradation decreases. If the fatty acids are removed from the medium, an immediate rise in population is noted. Total protein as measured by the method of Lowry has proved to be a reliable indicator of the microbial population and indirectly, the degradation of the saturate fraction. The analysis of total protein is superior in reliability and ease of assay to viable plate counts and dissolved oxygen measurements. In the systems employed, hexadecane has proved to be inadequate as a "model" hydrocarbon. Growth was very poor on n-hexadecane, and in cultures of P. oleovorans hexadecanoic acid was shownto accumulate.
Description
August 1976
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY