Shear flow experiments toward the development of a containerless bioreactor
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Authors
Rasheed, Fayaz
Issue Date
2018-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Aeronautical engineering
Alternative Title
Abstract
In the steady flow case, the non-dimensional velocity profiles showed very low dependence on Reynolds number. The oscillatory flow was vital since it enhances mixing, compared to a steady flow. The phase lag in the interfacial velocity near the knife edge in the oscillatory case, at various Reynolds and Stokes numbers, were measured. The phase lag became steeper with increasing forcing frequency and amplitude, and the response of the oscillatory forced system was found to be more sensitive compared to steady. The second set of experiments were performed involving interfacial shearing of protein solution contained in a cylindrical dish, again using a knife edge. Here the fibril formation of protein (human recombinant insulin) with shear and the decrease in concentration of native protein solution with time was studied. The ability of native protein monomers to transport to the interface and be adsorbed to the interface is found to be crucial to shear-induced fibrillization phenomenon. The knife edge protein shearing experiment serves as an analog to the ring-sheared drop experiment onboard the ISS, where the solution of protein in the dish has a similar surface area to volume ratio as the ring-sheared drop experiment. The results and conclusions made from this dissertation are a major step in understanding the science and behavior of fluids under interfacial shear for the development of a containerless bioreactor in space.
Description
August 2018
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY