Affinity precipitation of monoclonal antibodies using stimuli responsive smart biopolymers : methods development and process considerations
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Authors
Sheth, Rahul Dinesh
Issue Date
2014-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical and biological engineering
Alternative Title
Abstract
Finally, a proof of concept scalable mAb affinity precipitation process is developed using scaled down filtration techniques. Tangential flow filtration (TFF) is initially employed for the recovery of the precipitates formed during the process. While the precipitates formed by the ELP-Z-mAb complex can be recovered using TFF at high throughputs, the ELP-Z precipitates foul the membrane significantly. Confocal microscopy is used to gain further insights into the nature of precipitation. A normal flow filter train is then successfully employed for recovering the ELP-Z precipitates at high throughputs. The results presented in this thesis clearly demonstrate the potential of the ELP-Z based affinity precipitation process as a next generation unit operation for industrial mAb bioprocessing.
Description
May 2014
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY