Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II

Peng, Yanfei
Yu, Yanlei
Lin, Lei
Liu, Xinyue
Zhang, Xing
Wang, Peipei
Hoffman, Pauline
Kim, So Young
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II, Y. Peng, Y. Yu, L. Lin, X. Liu, X. Zhang, P. Wang, P. Hoffman, S.-Y. Kim, F. Zhang, R. J. Linhardt, Glycoconjugate Journal, 35, 119–128, 2018.
Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour. In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry. The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR). The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively. The disaccharide composition of CS consisted of 4S (49%), 0S (38%) 6S (12%), 2S6S (1.5%) and 2S4S (0.3%). The disaccharide composition of HS consisted of 0S (80%), NS2S (7%), NS (7%), 6S (4%), NS6S (2%), and TriS, 2S and 4S6S (each at 0.1%). The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively. SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE. Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.
Glycoconjugate Journal, 35, 119–128
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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Rensselaer Polytechnic Institute, Troy, NY
Glycoconjugate Journal
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