Cell free production of a liquid fuel

Authors
Grimaldi, Joseph
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Other Contributors
Belfort, Georges
Collins, Cynthia H.
Belfort, Marlene
Tessier, Peter M.
Kilduff, James
Issue Date
2014-12
Keywords
Chemical engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
Limitations on biofuel production using cell culture (Escherichia coli, Clostridium, Saccharomyces cerevisiae, brown microalgae, blue-green algae and others) include low product (alcohol) concentrations (2-5 vol%) due to feedback inhibition, instability of cells, and lack of economical product recovery processes. To overcome these challenges, we propose an alternate simplified biofuel production scheme based on a cell-free immobilized enzyme system. Optimized immobilized enzymes can be more stable and productive than free enzyme in solution. We measured the activity of two multi-domain enzymes, alcohol dehydrogenase (ADH) and keto-acid decarboxylase (KdcA), after immobilization onto multiple surface curvatures and chemistries. β-galactosidase was used as a model enzyme, with a colorimetric assay, to help develop an immobilization strategy. Several solid supports including silica nanoparticles (convex), mesopourous silica (concave), carbon diatoms (concave), and methacrylate (concave) were tested as a function of external/internal porosities.
Description
December 2014
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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