Driving arterial vascular differentiation of embryonic stem cells through neuropilin-1

Kim, Diana
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Dai, Guohao
Temple, Sally
Thompson, Deanna M.
Vincent, Peter
Wan, Leo Q.
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Biomedical engineering
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Characterization of the derived Nrp1+ endothelial progenitor cells (EPCs) reveal that they do indeed express higher levels of many arterial markers, in particular EphrinB2, and lower levels of venous marker, EphB4. Subsequent culture of these sorted populations on Matrigel revealed a highly migratory and network-building behavior of the Nrp1+ cells even in the absence of growth factors. Conversely, the Nrp1- EPCs showed almost no tube/cord formation. The sorted subpopulations were also exposed to fluid shear stress using an IBIDI flow chamber system and a cone-and-plate shear device. When the populations are exposed to shear stress for duration of 24 hours, they both respond by up-regulating EphrinB2 and down-regulating EphB4. However, the Nrp1+ EPCs demonstrated the potential to be more sensitive to mechanical stimuli, and this trend became more noticeable with longer duration of shear stress. In summary, the findings of this research bring a new perspective in isolating vascular progenitor cells; Nrp1+ vascular progenitor cells have the potential to be more responsive to inducers of arterial fate.
May 2016
School of Engineering
Dept. of Biomedical Engineering
Rensselaer Polytechnic Institute, Troy, NY
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