Comparison of low molecular weight heparins prepared from bovine heparins with enoxaparin
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Authors
Liu, Xinyue
Stange, Kalib
Fareed, Jawed
Hoppensteadt, Debra
Jeske, Walter
Kouta, Ahmed
Chi, Lianli
Jin, Caijuan
Jin, Yongsheng
Yao, Yiming
Issue Date
2017-09-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries. Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market. It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable. Bovine lung heparin is a potential alternative source for producing enoxaparin. Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed. Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy. The results suggested that the differences between PIEs and BLEs mainly result from N-acetylation differences derived from the parent heparins. In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays. We conclude that BLE has the potential to be developed as an analogue of PIE, although some challenges still remain.
Description
Clinical and Applied Thrombosis and Hemostasis, 23, 542-553
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Comparison of low molecular weight heparins prepared from bovine heparins with enoxaparin, X. Liu, K. St.Ange, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, L. Chi, C. Jin, Y. Jin, Y. Yao, R. J. Linhardt, Clinical and Applied Thrombosis and Hemostasis, 23, 542-553, 2017.
Publisher
Sage
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
19382723
10760296
10760296