Analysis of heparins derived from bovine tissues and comparison to porcine intestinal heparins
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Authors
St Ange, Kalib
Onishi, Akihiro
Fu, Li
Sun, Xiaojun
Lin, Lei
Mori, Daisuke
Zhang, Fuming
Dordick, Jonathan S.
Fareed, Jawed
Hoppensteadt, Debra
Issue Date
2016-09-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin is a widely used clinical anticoagulant. It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues. Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs. Pharmaceutical heparin currently used in the United States is primarily extracted from porcine intestines. Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin. These additional sources are intended to serve to diversify the heparin supply, making this lifesaving drug more secure. The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin. The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin. The in vitro functional activities of these heparins have also been determined. The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins. Porcine and bovine heparins have structural and compositional similarities and differences.
Description
Clinical and Applied Thrombosis and Hemostasis, 22, 520-527
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Analysis of heparins derived from bovine tissues and comparison to porcine intestinal heparins, K. St.Ange, A. Onishi, L. Fu, X. Sun, L. Lin, D. Mori, F. Zhang, J. S. Dordick, J. Fareed, D. Hoppensteadt, Walter Jeske, R. J. Linhardt, Clinical and Applied Thrombosis and Hemostasis, 22, 520-527, 2016.
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Journal
Volume
Issue
PubMed ID
DOI
ISSN
19382723
10760296
10760296