Metachromatic Activity of Heparin and Heparin Fragments

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Authors
Grant, Arthur C.
Linhardt, Robert J.
Fitzgerald, Gerald L.
Park, Janice J.
Langer, Robert
Issue Date
1984-02-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Heparin of an average molecular weight of 13,000 was fractionated on the basis of size into five fractions of different weight-average molecular weight ranging from 8500 to 20,000. The heparin was also degraded using microbial heparinase resulting in products ranging from a disaccharide of molecular weight 500 to an oligosaccharide of molecular weight 3100. These products were also size fractionated. The individual heparin fractions and products were tested for metachromatic activity with Azure A. The metachromatic activity of the heparin fractions was independent of molecular weight, while the metachromatic activity of the products was dependent on molecular weight. Metachromatic activity was found in a fragment as small as a tetrasaccharide. Anticoagulant activity was found in fragments of tetrasaccharide or larger by a Factor Xa clotting assay and in fragments of hexasaccharide or larger by a Factor Xa amidolytic chromogenic assay.
Description
Analytical Biochemistry, 137, 25-32
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Full Citation
Metachromatic Activity of Heparin and Heparin Fragments, A.C. Grant, R.J. Linhardt, G.. Fitzgerald, J.J. Park, R. Langer, Analytical Biochemistry, 137, 25-32 (1984).
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https://doi.org/10.1016/0003-2697(84)90341-5
 https://hdl.handle.net/20.500.13015/6030
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DOI
ISSN
10960309
32697
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Linhardt Research Labs Papers