Toward therapeutic applications using mesenchymal stem cells: bone defect repair and immunoregulation
Loading...
Authors
Arabiyat, Ahmad
Issue Date
2021-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biomedical engineering
Alternative Title
Abstract
This motivated the development of cell-free approaches to employ the therapeutic qualities of the MSC secretome sans the challenges associated with cell-based approaches. In these contexts, the overarching goals of this thesis are to: (1) evaluate the osteoinductive capacity of novel material-based approaches to bone defect repair; and (2) assess a pharmacologic strategy to enhance the immunoregulatory function of the MSC secretome towards the development of cell-free therapies. Towards the first goal, we evaluate and demonstrate the intrinsic osteoinductive capacity of two novel materials using bone marrow-derived human MSCs (h-MSCs): (1) poly(ε-caprolactone)-diacrylate (PCL-DA) shape memory polymers incorporating thermoplastic poly-L-lactic acid (PLLA) in a semi-interpenetrating design, and (2) incorporation of a phosphonated-siloxane macromer in a templated poly(ethylene glycol)-diacrylate hydrogel scaffolds.
Towards the second goal, we expand on the array of immune markers beyond what is typically measured from proinflammatory macrophages following treatment with native and cytokine-primed h-MSC conditioned-medium (CM). In addition, we demonstrate a combinatorial treatment modality utilizing purinergic P2 receptor antagonist suramin and h-MSC CM for transitioning macrophages towards a pro-regulatory and wound healing phenotype.
Description
May 2021
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY