Protein capture and identification at G-quadruplex DNA affinity surfaces
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Authors
Zhang, Tian
Issue Date
2013-12
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
The second part of this dissertation focuses on the fundamental characterization of affinity surfaces. Specifically, we studied affinity protein capture at G-quadruplex-forming-aptamer-modified surfaces using imaging tools. Two systems were used to investigate the DNA-modification and protein capture characteristics of different affinity surfaces: first, thrombin binding aptamer (TBA) and a control oligo sequence with no G-quadruplex forming ability, and second, the Pu28-mer sequence. The affinity surfaces of interest included fused silica plates, streptavidinated magnetic beads, and indium tin oxide (ITO)-coated glass slides. The results of these experiments provide a better fundamental understanding of protein capture at aptamer-modified surfaces that might lead to improved design and reliability of devices and processes for protein analysis, separation, isolation and purification.
Description
December 2013
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY