Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan

Zhang, Zhenqing
Xie, Jin
Liu, Jian
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan, Z. Zhang, J. Xie, J. Liu, R. J. Linhardt, Journal of American Society of Mass Spectrometry, 19, 82-89, 2008.
Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1→3 and in NAH 1→4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.
Journal of American Society of Mass Spectrometry, 19, 82-89
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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Rensselaer Polytechnic Institute, Troy, NY
Journal of the American Society for Mass Spectrometry