Biochemical and physical characterization of C-5 epimerase and 2-O-sulfotransferase
Loading...
Authors
Li, Kevin
Issue Date
2015-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical engineering
Alternative Title
Abstract
Enzymes are required in order to create bioengineered heparin. N-Sulfo heparosan (NS), a heparin-like structure, undergoes multiple enzymatic reactions to yield a heparin generic with similar chemical and biological activity. Two of the enzymes: C-5 epimerase and 2-O-sulfotransferase are used in the first step of the bioengineered heparin process. Unfortunately, this step gives low yields of the converted product (NS to NS2S) requiring a 1:1 substrate to enzyme concentration to see appreciable effects. Higher concentrations of enzyme are used to achieve yield that are deemed acceptable to move on subsequent enzymatic steps.
Description
May 2015
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY