Immuno-Affinity Purification of Heparinase
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Authors
Llnhardt, Robert J.
Merchant, Zohar M.
Persinger, David W.
Issue Date
1985-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Polyclonal IgG rabbit antibodies were prepared against a purified heparinase from Flavobacterium heparinum. Immuno-affinity purification of crude and partially purified heparinase is described. The resulting enzyme was of comparable purity to that prepared using the standard multistep purification scheme. The antibodies prepared were found to increase the activity of bound heparinase.
Description
The International Journal of Biochemistry, 17, 1179-1183
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Immuno-Affinity Purification of Heparinase, R.J. Linhardt, Z.M. Merchant, D.W. Persinger, The International Journal of Biochemistry, 17, 1179-1183 (1985).
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Volume
Issue
PubMed ID
DOI
ISSN
0020711X