Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin

Wang, Zhenyu
Li, Jennifer
Cheong, Samantha
Bhaskar, Ujjwal
Akihiro, Onishi
Zhang, Fuming
Dordick, Jonathan S.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin, Z. Wang, J. Li, S. Cheong, U. Bhaskar, O. Akihiro, F. Zhang, J.S. Dordick, R.J. Linhardt, Journal of Biotechnology, 156, 188-196, 2011.
The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from E. coli generated heparosan starting material of different molecular weights.
Journal of Biotechnology, 156, 188-196
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Biotechnology
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