Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin
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Authors
Wang, Zhenyu
Li, Jennifer
Cheong, Samantha
Bhaskar, Ujjwal
Akihiro, Onishi
Zhang, Fuming
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2011-12-10
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from E. coli generated heparosan starting material of different molecular weights.
Description
Journal of Biotechnology, 156, 188-196
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin, Z. Wang, J. Li, S. Cheong, U. Bhaskar, O. Akihiro, F. Zhang, J.S. Dordick, R.J. Linhardt, Journal of Biotechnology, 156, 188-196, 2011.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
18734863
1681656
1681656