Biochemical and physiological characterization of the three NADH dehydrogenases in Pseudomonas aeruginosa

Authors
Hreha, Teri N.
ORCID
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Other Contributors
Barquera, Blanca L.
Koffas, Mattheos A. G.
Makhatadze, George I.
Royer, Catherine Ann
Issue Date
2016-05
Keywords
Biology
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
This project examines the role of the NADH dehydrogenases in respiration and virulence in P. aeruginosa. We have found that all three of the NADH dehydrogenases (Na+-NQR, NUO and NDH2) are active during both exponential and stationary phases of growth, but are regulated differently, and do not compensate for each other when one is eliminated. Inactivating any of the NADH dehydrogenases produces different, but profound effects on virulence, pyocyanin production, biofilm formation, antibiotic resistance and NADH availability in the cell. This leads to the conclusion that regulation of the NADH dehydrogenases helps to control the onset of virulence and stationary phase-like behavior in P. aeruginosa.
Description
May 2016
School of Science
Department
Dept. of Biological Sciences
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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