Biochemical and physiological characterization of the three NADH dehydrogenases in Pseudomonas aeruginosa

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Authors
Hreha, Teri N.
Issue Date
2016-05
Type
Electronic thesis
Thesis
Language
ENG
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Biology
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Abstract
This project examines the role of the NADH dehydrogenases in respiration and virulence in P. aeruginosa. We have found that all three of the NADH dehydrogenases (Na+-NQR, NUO and NDH2) are active during both exponential and stationary phases of growth, but are regulated differently, and do not compensate for each other when one is eliminated. Inactivating any of the NADH dehydrogenases produces different, but profound effects on virulence, pyocyanin production, biofilm formation, antibiotic resistance and NADH availability in the cell. This leads to the conclusion that regulation of the NADH dehydrogenases helps to control the onset of virulence and stationary phase-like behavior in P. aeruginosa.
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May 2016
School of Science
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Rensselaer Polytechnic Institute, Troy, NY
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