Fractionation of Heparin-Derived Oligosaccharides by Gradient Polyacrylamide-Gel Electrophoresis
No Thumbnail Available
Authors
Rice, K.G.
Rottink, M.K.
Linhardt, Robert J.
Issue Date
1987-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known structure and having a degree of polymerization (dp) 2-6 were used to establish that the separation on gradient PAGE was primarily dependent on molecular size. High-Mr oligosaccharides (dp 8-20) were prepared using strong-anion-exchange h.p.l.c. and were used to help characterize the gradient PAGE separation. Kinetic profiles were obtained for the depolymerization of heparin and heparan sulphate with heparinase and heparitinase respectively. The utility of this approach in sequencing oligosaccharides derived from glycosaminoglycans is discussed.
Description
Biochemical Journal, 244, 515-522
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Fractionation of Heparin-Derived Oligosaccharides by Gradient Polyacrylamide-Gel Electrophoresis, K.G. Rice, M.K. Rottink, R.J. Linhardt, Biochemical Journal, 244, 515-522 (1987).
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
2646021