Biophysical characterization of seminal amyloid fibrils : interactions with gallic acid and green fluorescent protein
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Authors
LoRicco, Josephine Grace
Issue Date
2019-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biochemistry and biophysics
Alternative Title
Abstract
First, we explore a small molecule, gallic acid, that has potential to combat the fibril-enhanced transmission of HIV. We employ biophysical techniques to understand how gallic acid is able to reduce the infectivity of HIV in the presence of seminal plasma. Contrary to our initial hypothesis that gallic acid was able to disassemble mature seminal fibrils, we find that gallic acid binds to mature fibrils and modifies their surface properties rendering the fibrils less cationic. The cationic nature of the fibrils is necessary for enhancing infection. We also find that gallic acid is capable of inhibiting the aggregation of PAPf39 and SEM1(86-107) fibrils from monomeric peptide which could also contribute to reduction in infectivity in the presence of seminal amyloid fibrils.
Description
May 2019
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY