Purification of antibodies using elastin like polypeptide based affinity precipitation
Loading...
Authors
Bhat, Manish Krishna
Issue Date
2020-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical engineering
Alternative Title
Abstract
With the final aim being the development of a continuous affinity precipitation setup for the purification of mAbs, the first part deals with an improvement to the affinity precipitation process. Optimal conditions for the new elution without resolubilization process are identified by carrying out a high-throughput study in batch mode. We successfully obtain high recoveries above 90% for both the mAbs tested.
Description
August 2020
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY