N,N,N-trimethyl chitosan nanoparticles for the delivery of monoclonal antibodies against hepatocellular carcinoma cells

Vongchan, Preeyanat
Wutti-In, Yupanan
Sajomsang, Warayuth
Gonil, Pattarapond
Kothan, Suchart
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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N,N,N-trimethyl chitosan nanoparticles for the delivery of monoclonal antibodies against hepatocellular carcinoma cells, P. Vongchan, Y. Wutti-In, W. Sajomsang, P. Gonil, S. Kothan, R. J. Linhardt, Carbohydrate Polymers, 85, 215-220, 2011.
N,N,N-Trimethyl chitosan chloride is capable of forming nanocomplexes with protein through ionotropic gelation. A monoclonal antibody, raised against human liver heparan sulfate proteoglycan and specifically inhibiting hepatocellular carcinoma in vitro, was prepared in nanocomplexes of this modified chitosan. The smallest nanocomplexes (59 ± 17 nm, zeta-potential 16.5 ± 0.5 mV) were obtained at polysaccharide:antibody ratios of 5:0.3. Spherical particles with a smooth surface and compact structure having a mean diameter of ~11.2 ± 0.09 nm were investigated by Atomic Force Microscopy. Cellular uptake of fluorescently labeled nanocomplexes was studied in mouse monocyte models of cancer and normal cells. External and internal fluorescence was analyzed by flow cytometry. The results demonstrate that the nanocomplexes could enter cells and were retained for a longer period of time in cancer cells where they exhibited greater toxicity. These nanocomplexes appear safe and could potentially enhance the half-life of added antibodies.
Carbohydrate Polymers, 85, 215-220
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Polymers
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