Protease-catalyzed synthesis of peptide conjugates
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Authors
Centore, Robert
Issue Date
2020-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
In the first part of this thesis the polymerization of an oligo(Leu) segment from [poly(ethylene glycol) methyl ether]-phenylalanine ethyl ester to form mPEG-b-Phe(Leu)x diblock is described. By taking advantage of natural self-assembly occurring during the aqueous polymerization, a large decrease in the dispersity of the Leu segment in the diblock is observed along with control over the DP of the growing Leu segment. By studying the self-assembly of the reaction product, a mechanism was formulated where in situ co-assembly of mPEG-b-Phe(Leu)x and oligo(Leu)x co-product facilitates precipitation of a β-sheet co-assembly, preventing peptide hydrolysis and further polymerization, and thus providing a means to lower the dispersity and control the DP (DP = 5.1, dispersity < 1.02).
Description
May 2020
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY