Solvolytic depolymerization of chondroitin and dermatan sulfates
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Authors
Toida, Toshihiko
Sato, Kenji
Sakamoto, Noriko
Sakai, Shinobu
Hosoyama, Saori
Linhardt, Robert J.
Issue Date
2009-05-12
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
It is essential to establish a library of glycosaminoglycan oligosaccharides from the chondroitin and dermatan sulfates to investigate their biological functions and structure-activity relationships (SARs). There are several approaches to obtain oligosaccharides using chemical and enzymatic degradation procedures; however, purification of each resulting oligosaccharide is complicated because of the diversity of sulfonation patterns present in these oligosaccharides. We have developed a new method for the solvolytic degradation for chondroitin and dermatan sulfates to obtain an oligosaccharide mixture that can be easily purified into chondro/dermato oligosaccharides for characterization by both (1)H NMR and MALDI-TOFMS. These oligosaccharides have a methyl-esterified uronate residue and a methyl 2-acetamido-2-deoxy-D-galactofuranoside at the nonreducing and reducing ends, respectively. All other internal repeating disaccharide units were desulfonated, but maintained their core carbohydrate structures.
Description
Carbohydrate Research, 344, 888-893
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Solvolytic depolymerization of chondroitin and dermatan sulfates, T. Toida, K. Sato, N. Sakamoto, S. Sakai, S. Hosoyama, R. J. Linhardt, Carbohydrate Research, 344, 888-893, 2009.
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Terms of Use
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Volume
Issue
PubMed ID
DOI
ISSN
86215