Investigation of the effects of nucleotide mutations in the loop regions of the vegf gene promoter g-quadruplex sequence on its protein binding specificity

Authors
DeWitt, Dylan
ORCID
Loading...
Thumbnail Image
Other Contributors
McGown, Linda Baine
Lakshmi, K. V.
Linhardt, Robert J.
Issue Date
2020-05
Keywords
Chemistry
Degree
MS
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
This project focuses on the investigation of the effects on binding specificity of mutations in the VEGF promoter sequence that was identified to bind to a few nuclear proteins. The VEGF promoter was previously discovered by our group to be a promising potential aptamer using our genome-inspired reverse selection approach to aptamer discovery. The goal of this project was to learn more about the effects of sequence mutations on the binding specificity of the VEGF promoter. These studies demonstrate that nucleotide base mutations in the loop regions of the VEGF promoter can improve its specificity and reveals the potential of future research into the mutation of aptamer sequences.
Description
May 2020
School of Science
Department
Dept. of Chemistry and Chemical Biology
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
Access
Restricted to current Rensselaer faculty, staff and students. Access inquiries may be directed to the Rensselaer Libraries.