Isolation of a lectin binding rhamnogalacturonan-I containing pectin from pumpkin

Zhao, J.
Zhang, F.
Liu, X.
St.Ange, K.
Zhang, A.
Li, Q.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Isolation of a lectin binding rhamnogalacturonan-I containing pectin from pumpkin, J. Zhao, F. Zhang, X. Liu, K. St.Ange, A. Zhang, Q. Li, R. J. Linhardt, Carbohydrate Polymers, 163, 330–336, 2017.
A rhamnogalacturonan-I (RG-I) containing pectic polysaccharide (PPc) was isolated from pumpkin following a low-temperature alkali treatment and a combination of gradual alcohol precipitation and ion-exchange. Monosaccharide compositional analysis of PPc revealed the presence of rhamnose, galacturonic acid, galactose, and arabinose in a molar ratio of 7.4: 25: 28: 2.6. Structural and linkage analysis by 1D NMR (1H NMR and 13C NMR), and 2D NMR (COSY, TOCSY, HSQC, and elevated temperature HMBC) suggested that PPc was a RG-I-like pectic polysaccharide, branched at the C-4 of some of the (about 29% of) rhamnosyl units, with relatively long β-1,4-d-galactan side chains to which were attached, through the C-3 of β-d-Gal, terminal non reducing α-Araf units. The results of surface plasmon resonance (SPR) show that PPc binds to two types of lectin, Ricinus communis agglutinin 120 (RCA120) and Galectin-3 (Gal-3). These binding studies show quick association and slow dissociation with a moderate binding affinity between PPc and Gal-3 of 1.26μM. The interaction between PPc and Gal-3 suggest the potential use of pumpkin pectic polysaccharide as a Gal-3 inhibitor in functional food or drug development applications.
Carbohydrate Polymers, 163, 330–336
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