Microbial co-culture conversion of lignocellulosic sugars to high-value chemicals
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Authors
Gedeon, Kamil Sankara
Issue Date
2019-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical engineering
Alternative Title
Abstract
The final aim of this work was to engineer co-cultures for improved product titer and increased total sugar consumption as compared to mono-cultures. From this work we showed violacein production solely xylose for the first time in E. coli. We also showed the production of violacein and naringenin from co-cultures in mixed sugar media. By varying inoculation ratios of the two E. coli strains, we successfully determined co-culture conditions that resulted in higher production of violacein and naringenin than their mono-culture counterparts. The final co-culture system serves as a platform for improved production of other biomolecules from lignocellulosic sugars over mono-culture production, while demonstrating that modularization of metabolic pathways in different strains can improve product titer, and sugar consumption.
Description
August 2019
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY