Identification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis
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Authors
Higashi, K.
Takeda, Keita
Mukuno, A.
Okamoto, Y.
Masuko, S.
Linhardt, Robert J.
Toida, T.
Issue Date
2016
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Gal) in the case of KS]. Among the GAGs, CS shows structural variations, such as sulfation patterns and fucosylation, which are responsible for their physiological functions through CS interaction with CS-binding proteins. Here, we solved the structure of KS-branched CS-E derived from a clam, Mactra chinensis. KS disaccharide [d-GlcNAc6S-(1→3)-β-d-Gal-(1→] was attached to the C-3 position of GlcA, and consecutive KS-branched disaccharide sequences were found in a CS chain. KS-branched polysaccharides clearly exhibited resistance to degradation by chondroitinase ABC or ACII (at low concentrations) compared with typical CS structures. Furthermore, KS-branched polysaccharides stimulated neurite outgrowth of hippocampal neurons. These results strongly suggest that M. chinensis is a rich source of KS-branched CS, and it has important biological activities.
Description
Biochemical Journal, 473 4145–4158
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Identification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis, K. Higashi, K. Takeda, A. Mukuno, Y. Okamoto, S. Masuko, R. J. Linhardt, T. Toida, Biochemical Journal, 473 4145–4158, 2016.
Publisher
Portland Press Limited