Wide-field macroscopic fluorescence lifetime imaging for pre-clinical assessment of targeted drug delivery
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Authors
Sinsuebphon, Nattawut
Issue Date
2018-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biomedical engineering
Alternative Title
Abstract
This study optimized our wide-field MFLI FRET imaging for preclinical applications by selecting the best near-infrared (NIR) FRET pair and implementing reflectance imaging geometry. Comparison of five NIR FRET pairs using a cell-based assay revealed that Alexa Fluor 700 and Alexa Fluor 750 was the best NIR pair due to its brightness and a good relationship between FRET level and acceptor to donor ratios. Implementation of reflectance illumination geometry allowed whole-body imaging of small animals. The validation was performed with the previously implemented transmission illumination geometry. There was no difference between lifetime-based FRET quantification in vitro and in vivo.
Description
August 2018
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY