Isolation and structural characterization of glycosaminoglycans from heads of red salmon (Oncorhynchus nerka)
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Authors
Zhang, F.
Xie, J.
Linhardt, Robert J.
Issue Date
2014
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycosaminoglycans (GAGs) are linear, highly negatively charged polysaccharides. They are ubiquitous molecules exhibiting a wide range of biological functions with numerous applications in pharmaceutical, cosmetic, and nutraceutical industrials. The commercial fish-processing industry generates large quantities of solid waste, which can represent a potential resource for GAG production. In this study, we used a three-step recovery and purification scheme for isolation of GAGs from the heads of red salmon (Oncorhynchus nerka). The GAGs recovery yield was 6 to 7 mg from 1 gram of salmon head powder. The recovered GAGs were structurally analyzed with polyacrylamide gel electrophoresis and by disaccharide composition analysis with reversed-phase ion-pair high-performance liquid chromatography. The analyses showed the major composition of the GAGs in red salmon head were chondroitin sulfate C and E.
Description
Journal of Biotechnology and Bioengineering, 1, 1-5
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Isolation and structural characterization of glycosaminoglycans from heads of red salmon (Oncorhynchus nerka), F. Zhang, J. Xie, R. J. Linhardt, Journal of Biotechnology and Bioengineering, 1, 1-5, 2014.
Publisher
Jacobs