Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Liu, J.
Wang, Z.
Arnold, K.
Xu, Y.
Pagadala, V.
Su, G.
Myatt, H.
Linhardt, Robert J.
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Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering , Biology
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Attribution 3.0 United States
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Developing a quantitative method to analyze heparan sulfate using isotopically labeled calibrants, Z. Wang, K. Arnold, Y. Xu, V. Pagadala, G. Su, H. Myatt, R.J. Linhardt, J. Liu, Communications Biology, 3, 425, 2020.
Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
Communications Biology, 3, 425
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Rensselaer Polytechnic Institute, Troy, NY
The Linhardt Research Labs Online Collection
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