Quantitative analysis of heparan sulfate using isotopically labeled calibrants
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Authors
Liu, J.
Wang, Z.
Arnold, K.
Xu, Y.
Pagadala, V.
Su, G.
Myatt, H.
Linhardt, Robert J.
Issue Date
2020
Type
Article
Language
ENG
Keywords
Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering , Biology
Alternative Title
Abstract
Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
Description
Communications Biology, 3, 425
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Developing a quantitative method to analyze heparan sulfate using isotopically labeled calibrants, Z. Wang, K. Arnold, Y. Xu, V. Pagadala, G. Su, H. Myatt, R.J. Linhardt, J. Liu, Communications Biology, 3, 425, 2020.