Sulfonation of papain treated chitosan and its mechanism for anticoagulant activity
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Authors
Suwan, Jiraporn
Zhang, Zhenqing
Li, Boyangzi
Vongchan, Preeyanat
Meepowpan, Puttinan
Zhang, Fuming
Mousa, Shaker A.
Mousa, Shaymaa
Premanode, Bhusana
Kongtawelert, Prachya
Issue Date
2009-07-06
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The novel low molecular weight chitosan polysulfate (MW 5,120–26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N, N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight.
Description
Carbohydrate Research, 344, 1190–1196
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Sulfonation of papain treated chitosan and its mechanism for anticoagulant activity, J. Suwan, Z. Zhang, B. Li, P. Vongchan, P. Meepowpan, F. Zhang, S.A. Mousa, S. Mousa, B. Premanode, P. Kongtawelert, R. J. Linhardt, Carbohydrate Research, 344, 1190–1196, 2009.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
86215