Red algal sulfated galactan binds and protects neural cells from HIV-1 gp120 and Tat
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Authors
Pomin, Vitor H.
Mahdi, Fakhri
Jin, Weihua
Zhang, Fuming
Linhardt, Robert J.
Paris, Jason J.
Issue Date
2021-08-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The potential neuroprotective capacity of four different sulfated glycans: Botryocladia occidentalis-derived sulfated galactan (BoSG) (MW > 100 kDa), Lytechinus variegatus-derived sulfated fucan (LvSF) (MW~90 kDa), high-molecular weight dextran sulfate (DxS) (MW 100 kDa), and unfractionated heparin (UFH) (MW~15 kDa), was assessed in response to the HIV-1 proteins, R5-tropic glycoprotein 120 (gp120) and/or trans-activator of transcription (Tat), using primary murine neurons co-cultured with mixed glia. Compared to control-treated cells in which HIV-1 proteins alone or combined were neurotoxic, BoSG was, among the four tested sulfated glycans, the only one capable of showing significant concentration-dependent neuroprotection against Tat and/or gp120, alone or combined. Surface plasmon resonance-based data indicate that BoSG can bind both HIV-1 proteins at nM concentrations with preference for Tat (7.5 × 10-8 M) over gp120 (3.2 × 10-7 M) as compared to UFH, which bound gp120 (8.7 × 10-7 M) over Tat (5.7 × 10-6 M). Overall, these data support the notion that sulfated glycan extracted from the red alga B. occidentalis, BoSG, can exert neuroprotection against HIV-1 Tat and gp120, potentially via direct molecular interactions.
Description
Pharmaceuticals, 14, 714
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Red algal sulfated galactan binds and protects neural cells from HIV-1 gp120 and Tat, V. H. Pomin, F. Mahdi, W. Jin, F. Zhang, R. J. Linhardt, J. J. Paris, Pharmaceuticals, 14, 714, 2021.
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PubMed ID
DOI
ISSN
14248247