Red algal sulfated galactan binds and protects neural cells from HIV-1 gp120 and Tat

Authors
Pomin, Vitor H.
Mahdi, Fakhri
Jin, Weihua
Zhang, Fuming
Linhardt, Robert J.
Paris, Jason J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2021-08-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Attribution 3.0 United States
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Full Citation
Red algal sulfated galactan binds and protects neural cells from HIV-1 gp120 and Tat, V. H. Pomin, F. Mahdi, W. Jin, F. Zhang, R. J. Linhardt, J. J. Paris, Pharmaceuticals, 14, 714, 2021.
Abstract
The potential neuroprotective capacity of four different sulfated glycans: Botryocladia occidentalis-derived sulfated galactan (BoSG) (MW > 100 kDa), Lytechinus variegatus-derived sulfated fucan (LvSF) (MW~90 kDa), high-molecular weight dextran sulfate (DxS) (MW 100 kDa), and unfractionated heparin (UFH) (MW~15 kDa), was assessed in response to the HIV-1 proteins, R5-tropic glycoprotein 120 (gp120) and/or trans-activator of transcription (Tat), using primary murine neurons co-cultured with mixed glia. Compared to control-treated cells in which HIV-1 proteins alone or combined were neurotoxic, BoSG was, among the four tested sulfated glycans, the only one capable of showing significant concentration-dependent neuroprotection against Tat and/or gp120, alone or combined. Surface plasmon resonance-based data indicate that BoSG can bind both HIV-1 proteins at nM concentrations with preference for Tat (7.5 × 10-8 M) over gp120 (3.2 × 10-7 M) as compared to UFH, which bound gp120 (8.7 × 10-7 M) over Tat (5.7 × 10-6 M). Overall, these data support the notion that sulfated glycan extracted from the red alga B. occidentalis, BoSG, can exert neuroprotection against HIV-1 Tat and gp120, potentially via direct molecular interactions.
Description
Pharmaceuticals, 14, 714
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
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The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Pharmaceuticals
https://harc.rpi.edu/
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Open Access
CC BY — Creative Commons Attribution